Published on 13 October 2013 by Moof University

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In this video, I discuss how the Lineweaver-Burk (or Double-Reciprocal) Plot works in association with hyperbolic equation and Michaelis-Menton kinetics.

Hyperbolas, when it comes to math, are not simple to work with, which is why the hyperbolic equation relating to Michaelis-Menton kinetics is altered to give the Lineweaver Burk equation / Double Reciprocal plot. It basically turns the hyperbolic equation into a linear equation, which is much easier to work with. The graph becomes 1/V plotted versus 1/substrate concentration. The x intercept is equal to -1/Km, and the y intercept is equal to 1/Vmax, which makes it easy to calculate for either when the values for the intercepts are available. The slope of the line is equal to Km/Vmax.

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